Exosomes are nanosized extracellular vesicles (30–150 nm) secreted by cells, including Mesenchymal Stem Cells (MSCs). They serve as intercellular messengers, delivering proteins, lipids, mRNA, and microRNA to target cells, and influencing a wide range of physiological and regenerative processes.
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Collect culture media containing exosomes.
Remove cells and debris via sequential spins.
Add Polymerto promote exosome aggregation.
Incubate to allow exosome precipitation.
Pellet exosomes by centrifugation.
Clean pellet and resuspend in buffer for testing or -80°C storage.
Page 2 of 19Saleem et al. Stem Cell Research & Therapy (2024) 15:477they have considerable latent drug delivery systems forthe
Exosome therapy is a burgeoning field of regenerative medicine that utilizes the potential of exosomes, tiny extracellular vesicles, to facilitate
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y genes involved in the key pathways of β-catenin, WNT, BMP2 and BMP4 promotes the proliferation of hair follicle stem
Researchers use lab-grown cells, rats to better understand disease mechanisms Margarida Maia, PhD avatar by Margarida Maia, PhD | July
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